A one-step cobalt-ferrocyanide method for histochemical demonstration of acetylcholinesterase activity in central nervous system tissue.

نویسندگان

  • C R Schatz
  • C Geula
  • R J Morecraft
  • M M Mesulam
چکیده

We introduce a one-step histochemical method with cobalt as the precipitating agent for ferrocyanide for the light microscopic demonstration of acetylcholinesterase activity. This method was used to demonstrate acetylcholinesterase in normal cortical fibers and neurons, as well as pathological elements such as plaques and tangles. This procedure can also be easily combined with immunohistochemical methods that use diaminobenzidine as a chromogen.

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عنوان ژورنال:
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

دوره 40 3  شماره 

صفحات  -

تاریخ انتشار 1992